Application
WB analysis of HeLa lysate using PEG10 antibody at a dilution of 1:1,000.Application
IP analysis of HeLa lysates using control IgG and PEG10 antibody. The precipitates were detected by the same PEG10 antibody.| Product Name | PEG10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [1E2-F12-C12] to PEG10This is a paternally expressed imprinted gene that encodes transcripts containing two overlapping open reading frames (ORFs), RF1 and RF1/RF2, as well as retroviral-like slippage and pseudoknot elements, which can induce a -1 nucleotide frame-shift. ORF1 encodes a shorter isoform with a CCHC-type zinc finger motif containing a sequence characteristic of gag proteins of most retroviruses and some retrotransposons. The longer isoform is the result of -1 translational frame-shifting leading to translation of a gag/pol-like protein combining RF1 and RF2. It contains the active-site consensus sequence of the protease domain of pol proteins. Additional isoforms resulting from alternatively spliced transcript variants, as well as from use of upstream non-AUG (CUG) start codon, have been reported for this gene. Increased expression of this gene is associated with hepatocellular carcinomas. [provided by RefSeq, May 2010] |
| Immunogen | Purified recombinant human PEG10 protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1E2-F12-C12 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Mart2 RGAG3 MEF3L Mar2 paternally expressed 10 HB-1 EDR |
|---|---|
| Target | PEG10 |
| Molecular Weight(MW) | 55kDa (note) |
| Cellular Localization | Nucleus. Cytoplasm |
Application
WB analysis of HeLa lysate using PEG10 antibody at a dilution of 1:1,000.
Application
IP analysis of HeLa lysates using control IgG and PEG10 antibody. The precipitates were detected by the same PEG10 antibody.| Application Notes | IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol |
