| Product Name | Perforin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [CE2.10] to PerforinPerforin is one of the major cytolytic proteins of cytolytic granules. One of the main pathways of lymphocyte mediated cytolysis entails the secretion onto target membranes of cytolytic granules contained in cytolytic effector lymphocytes of T-cell or NK-cell type. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Perforin (PRF) is involved in the killing mediated by cytotoxic lymphocytes which represents an important mechanism in the immune defense against tumors and virus infections. Human PRF is a 555 amino acid protein with a 21 amino acid signal peptide. It has a molecular weight of 70 to 75 kD. PRF is a pore forming protein with a mechanism of transmembrane channel formation similar to C9 and homology between perforin and C9 has been demonstrated at their respective functionally conserved regions. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested. |
| Immunogen | Recombinant fragment, corresponding to amino acids 98 - 534 of Mouse Perforin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | CE2.10 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| IP: Use at an assay dependent dilution on human and mouse samples. WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa) from the mouse T cell clone B6.1. The antibody does not detect human perforin by Western blot. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Pfn Pfp Prf-1 Prf 1 Prf1 |
|---|---|
| Target | Perforin |
| Cellular Localization | Cytoplasmic granules |
| Positive Control | Mouse T cell clone B6.1 |
|---|---|
| Application Notes | IP: Use at an assay dependent dilution on human and mouse samples. WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa) from the mouse T cell clone B6.1. The antibody does not detect human perforin by Western blot. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
