Application
Flow cytometric Analysis of Hela cells, using anti-PFN1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
Flow cytometric Analysis of Jurkat cells, using anti-PFN1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
HEK293T cells transfected with either RC202338 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PFN1 antibody, and then analyzed by flow cytometry.Application
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-PFN1 mouse monoclonal antibody. ( Dilution 1:50)Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PFN1 monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PFN1.Application
Anti-PFN1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PFN1Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PFN1 mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PFN1 mouse monoclonal antibody. ( Dilution 1:50)| Product Name | PFN1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2D2] to PFN1Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. |
| Immunogen | Full length human recombinant protein of human PFN1 (NP_005013) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2D2 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| IHC 1:50 : |
|
| Species Reactivity | HumanDogMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | PFN1 |
|---|---|
| Tissue Specificity | is specific for human PFN1 (NP_005013). |
Application
Flow cytometric Analysis of Hela cells, using anti-PFN1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
Flow cytometric Analysis of Jurkat cells, using anti-PFN1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
HEK293T cells transfected with either RC202338 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PFN1 antibody, and then analyzed by flow cytometry.
Application
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-PFN1 mouse monoclonal antibody. ( Dilution 1:50)
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PFN1 monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PFN1.
Application
Anti-PFN1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PFN1
Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PFN1 mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PFN1 mouse monoclonal antibody. ( Dilution 1:50)| Application Notes | IHC 1:50 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
