Application
PGP9.5 antibody detects PGP9.5 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: PGP9.5 protein stained by PGP9.5 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .Application
PGP9.5 antibody detects PGP9.5 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PGP9.5 protein stained by PGP9.5 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
IHC analysis of porcine distal colon using PGP9.5 antibody (red). Abbreviations: Cr, colonic crypt; L, intestinal lumen; SM, submucosa. Scale bars: 100 μm.| Product Name | PGP9.5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PGP9.5 - Neuronal MarkerUCHL1 / Protein gene product 9.5(PGP 9.5) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons. [Taken from OMIM]. |
| Immunogen | Synthetic peptide: ASSEDTLLKDAAKVCR, corresponding to amino acids 176 - 191 of Human PGP9.5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-FWB |
| For IHC-Fr: Use at a dilution of 1:100-1:1000. For WB: Use at a dilution of 1:1000. For ICC/IF: Use at a dilution of 1:100-1:1000. Predicted molecular weight: 26.8 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PARK5 PGP 9.5 PGP9.5 PGP95 Uch-L1 Uch L1 UchL1 |
|---|---|
| Target | PGP9.5 |
| Cellular Localization | Cytoplasmic |
Application
PGP9.5 antibody detects PGP9.5 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: PGP9.5 protein stained by PGP9.5 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
Application
PGP9.5 antibody detects PGP9.5 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PGP9.5 protein stained by PGP9.5 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
IHC analysis of porcine distal colon using PGP9.5 antibody (red). Abbreviations: Cr, colonic crypt; L, intestinal lumen; SM, submucosa. Scale bars: 100 μm.| Positive Control | Human brain lysate |
|---|---|
| Application Notes | For IHC-Fr: Use at a dilution of 1:100-1:1000. For WB: Use at a dilution of 1:1000. For ICC/IF: Use at a dilution of 1:100-1:1000. Predicted molecular weight: 26.8 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Containing 0.05% sodium azide |
