Application
Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 24k UCHL1 protein using UCHL1 antibody [BH7]Application
Rat spinal cord stained with anti-UCHL1(red) and anti-neurofilament NF-H antibody (green). The large cells are α-motorneurons and UCHL1 fills the cytoplasm of their perikarya and dendrites.| Product Name | PGP9.5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [BH7] to PGP9.5Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. |
| Immunogen | Recombinant full length human UCHL1 purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | BH7 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| ICC/IF:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanRatBovine |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | PARK5 PGP9.5 UCHL1 |
|---|---|
| Target | PGP9.5 |
| Molecular Weight(MW) | 24 (note) |
Application
Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 24k UCHL1 protein using UCHL1 antibody [BH7]
Application
Rat spinal cord stained with anti-UCHL1(red) and anti-neurofilament NF-H antibody (green). The large cells are α-motorneurons and UCHL1 fills the cytoplasm of their perikarya and dendrites.| Application Notes | ICC/IF:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long-term storage, aliquot and store at -20 C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS containing 10 mM sodium azide |
