| Product Name | PGP9.5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [13C4] to PGP9.5Protein gene product 9.5 (PGP9.5) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of PGP9.5 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons. |
| Immunogen | Native protein from brain. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 13C4 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-P |
| ELISA: 1/160000. Native protein coated at 100ng/well. IHC-P: 1/50 - 1/400. Fixation in 95% ethanol/5% acetic acid for 2-3 hours prior to paraffin embedding is recommended. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanPigRatGuinea Pig |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | Protein gene product9.5 Parkinson Disease 5 Ubiquiti Ubiquitin C terminal hydrolase (neuron specific) Ubiquitin carboxyl terminal hydrolase isozyme L1 Gracile axonal dystrophy Neuron cytoplasmic protein 9.5 PGP 9.5 Protein gene product 9.5 Parkins |
|---|---|
| Target | PGP9.5 |
| Tissue Specificity | Stains neuronal cell bodies and axons in the CNS and periphery, small nerve fibers in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas and tumors of the DNES. Also stains neuroendocrine cells in human adult gut. |
| Cellular Localization | Cytoplasmic |
| Application Notes | ELISA: 1/160000. Native protein coated at 100ng/well. IHC-P: 1/50 - 1/400. Fixation in 95% ethanol/5% acetic acid for 2-3 hours prior to paraffin embedding is recommended. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide. Constituents: PBS. pH 7.2 |
