Application
WB analysis of U251, Raji and IMP32 lysates using PGP9.5 antibody at a dilution of 1:1,000.Application
ICC/IF analysis of COS7 cells using PGP9.5 antibody at a dilution of 1:300.| Product Name | PGP9.5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [7B8-G5-E7] to PGP9.5The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease. |
| Immunogen | Purified recombinant human PGP9.5 protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 7B8-G5-E7 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) PGP 9.5 PARK5 PGP95 Uch-L1 UCHL1 PGP9.5 |
|---|---|
| Target | UCHL1 / PGP9.5 |
| Molecular Weight(MW) | 25kDa (note) |
| Cellular Localization | Cytoplasm |
Application
WB analysis of U251, Raji and IMP32 lysates using PGP9.5 antibody at a dilution of 1:1,000.
Application
ICC/IF analysis of COS7 cells using PGP9.5 antibody at a dilution of 1:300.| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide, 50% glycerol, 0.1%BSA |
