Application
Immunocytochemical staining of PHAP in Raji cells with PHAP antibody at 1μg/ml.Application
Western blot analysis of PHAP in Raji cell lysate with PHAP antibody at 1μg/ml. The wide and strong band below PHAP III is a doublet composed of PHAP I (upper) and PHAP I2a (lower).| Product Name | PHAP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PHAPThis antibody recognizes all three identified isoforms of human, mouse and rat PHAP, including PHAP I, PHAP I2a, and PHAP III. The PHAP proteins(tumor suppressor putative HLA-DR associated proteins) are important regulators of mitochondrial apoptosis. PHAP facilitates apoptosome-mediated caspase-9 activation to stimulate the mitochondrial apoptosis pathway. In addition, PHAP opposes both Ras- and myc- mediated cell transformation.Apoptosis is related to many diseases and development. Caspase 9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf 1, which forms an apoptosome that in turn binds to and activate procaspase 9. Activated caspase 9 cleaves and activates the effector caspases (caspase 3, 6 and 7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase 9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation. |
| Immunogen | Synthetic peptide (Human) (N terminal). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| For WB: Use at a concentration of 0.5 - 2 μg/ml. Detects a band of approximately 35 kDa. Using Raji cell lysate as a positive control, a band of approximately 35kDa is detected corresponding to PHAP III with a doublet below PHAP III correspoinging to PHAP I (upper band) and PHAP I2a (lower band). Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRatBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PHAP1 LANP PHAP C15orf1 I1PP2A MAPM MGC119787 MGC150373 PHAPI PP32 |
|---|---|
| Target | PHAP |
| Tissue Specificity | Using Raji cell lysate as a positive control, a band of approximately 35kDa is detected corresponding to PHAP III with a doublet below PHAP III correspoinging to PHAP I (upper band) and PHAP I2a (lower band). |
| Cellular Localization | Nuclear |
Application
Immunocytochemical staining of PHAP in Raji cells with PHAP antibody at 1μg/ml.
Application
Western blot analysis of PHAP in Raji cell lysate with PHAP antibody at 1μg/ml. The wide and strong band below PHAP III is a doublet composed of PHAP I (upper) and PHAP I2a (lower).| Positive Control | Raji cell lysate |
|---|---|
| Application Notes | For WB: Use at a concentration of 0.5 - 2 μg/ml. Detects a band of approximately 35 kDa. Using Raji cell lysate as a positive control, a band of approximately 35kDa is detected corresponding to PHAP III with a doublet below PHAP III correspoinging to PHAP I (upper band) and PHAP I2a (lower band). Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | PBS, pH 7.4, containing 0.02% sodium azide |
