Application
WB analysis of mouse kidney lysate using PHD2 antibody.Application
ICC/IF analysis of HeLa cells using PHD2 antibody at a dilution of 1:500 (green) and tubulin antibody (red). Nuclei were counterstained with DAPI (blue).Application
IHC analysis of renal tubular epithelium in mouse kidney cortex using PHD2 antibody at a concentration of 2.5 ug/ml.| Product Name | PHD2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to HIF Prolyl Hydroxylase 2HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. |
| Immunogen | Synthetic peptide: Internal portion of mouse PHD2 between residues 300-400 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PIPWB |
| For IHC-P: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AI503754 C1orf12 Hif-p4h-2 ORF13 Phd2 SM-20 SM 20 Hif p4h 2 SM20 Hifp4h2 |
|---|---|
| Target | HIF Prolyl Hydroxylase 2 |
Application
WB analysis of mouse kidney lysate using PHD2 antibody.
Application
ICC/IF analysis of HeLa cells using PHD2 antibody at a dilution of 1:500 (green) and tubulin antibody (red). Nuclei were counterstained with DAPI (blue).
Application
IHC analysis of renal tubular epithelium in mouse kidney cortex using PHD2 antibody at a concentration of 2.5 ug/ml.| Positive Control | Mouse kidney lysate |
|---|---|
| Application Notes | For IHC-P: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Tris-citrate/phosphate pH 7-8 containing 0.1% sodium azide |
