Application
Flow Cytometry: HIF Prolyl Hydroxylase 2 Antibody - Flow cytometric detection of PHD2, 10^6 Jurkat cells were fixed, permeabilized, and stained with 3.0 ug/ml anti-PHD2 in a 150 ul reaction.Application
'Western Blot: HIF Prolyl Hydroxylase 2 Antibody - Western blot analysis of human PHD2, using . Samples: Recombinant FLAG-His-PHD1, PHD2 and PHD3 (10 ng/lane), HeLa whole cell lysate and MEFs'Application
'Western Blot: HIF Prolyl Hydroxylase 2 Antibody - Detection of Human PHD2 by Western Blot. Samples: Recombinant epitope-tagged PHD1, PHD2 or PHD3 (10 ng/lane) or whole cell lysate from HeLa cells, stained with rabbit anti-PHD2 used at 1 ug/ml.'| Product Name | PHD2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PHD2HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. |
| Immunogen | A synthetic peptide made to an internal fragment of the human protein sequence of HIF prolyl hydroxylase 2. (residues 350-426) [LocusLink ID 54583] |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| WB: Use at a dilution of 1/500 - 1/1000 (ECL), a band is at 45-50 kDa, representing HIF prolyl hydroxylase 2. This antibody has been tested in human glioblastoma cell lysates. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C1orf12 DKFZp761F179 ECYT3 HIFPH2 HPH2 PHD2 SM20 ZMYND6 |
|---|---|
| Target | PHD2 |
| Tissue Specificity | This antibody is specific for HIF prolyl hydroxylase 2. |
| Cellular Localization | Cytoplasmic |
Application
Flow Cytometry: HIF Prolyl Hydroxylase 2 Antibody - Flow cytometric detection of PHD2, 10^6 Jurkat cells were fixed, permeabilized, and stained with 3.0 ug/ml anti-PHD2 in a 150 ul reaction.
Application
'Western Blot: HIF Prolyl Hydroxylase 2 Antibody - Western blot analysis of human PHD2, using . Samples: Recombinant FLAG-His-PHD1, PHD2 and PHD3 (10 ng/lane), HeLa whole cell lysate and MEFs'
Application
'Western Blot: HIF Prolyl Hydroxylase 2 Antibody - Detection of Human PHD2 by Western Blot. Samples: Recombinant epitope-tagged PHD1, PHD2 or PHD3 (10 ng/lane) or whole cell lysate from HeLa cells, stained with rabbit anti-PHD2 used at 1 ug/ml.'| Positive Control | Human glioblastoma cell lysates |
|---|---|
| Application Notes | WB: Use at a dilution of 1/500 - 1/1000 (ECL), a band is at 45-50 kDa, representing HIF prolyl hydroxylase 2. This antibody has been tested in human glioblastoma cell lysates. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | Preservative: 0.01% Sodium Azide; Constituents: Tris-citrate/phosphate (pH 7-8) |
