WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was incubated with anti-EGLN1 antibody at a dilution of 1:50000. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human kidney was stained with anti-EGLN1 Rabbit antibody.| Product Name | EGLN1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:10000-1:50000 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | EGLN1 |
|---|---|
| Gene Synonyms | HPH2 PHD2 SM20 ECYT3 HALAH HPH-2 HIFPH2 ZMYND6 C1orf12 HIF-PH2 |
| Gene Full Name | egl-9 family hypoxia inducible factor 1 |
| Gene Summary | The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). [provided by RefSeq, Nov 2009] |
| Molecular Weight(MW) | 46 kDa(Observed band size: 50 kDa) |
| Cellular Localization | Cytoplasm . Nucleus . Mainly cytoplasmic. Shuttles between the nucleus and cytoplasm (PubMed:19631610) . Nuclear export requires functional XPO1. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was incubated with anti-EGLN1 antibody at a dilution of 1:50000. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human kidney was stained with anti-EGLN1 Rabbit antibody.| Application Notes | WB:1:10000-1:50000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
