WB
Western blot analysis of various lysates using PHD2/EGLN1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.IHC
Immunohistochemistry analysis of paraffin embedded Rat kidney using PHD2/EGLN1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.ICC/IF
Immunofluorescence analysis of L929 cells using PHD2/EGLN1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.| Product Name | PHD2/EGLN1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-426 of human PHD2/EGLN1 (NP_071334.1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:100 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EGLN1 |
|---|---|
| Gene Synonyms | HPH2 PHD2 SM20 ECYT3 HALAH HPH-2 HIFPH2 ZMYND6 C1orf12 HIF-PH2 |
| Gene Full Name | egl-9 family hypoxia inducible factor 1 |
| Gene Summary | The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). [provided by RefSeq, Nov 2009] |
| Molecular Weight(MW) | 45kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Western blot analysis of various lysates using PHD2/EGLN1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
IHC
Immunohistochemistry analysis of paraffin embedded Rat kidney using PHD2/EGLN1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ICC/IF
Immunofluorescence analysis of L929 cells using PHD2/EGLN1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:100 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
