WB
Western blot analysis of PHD2 on different lysates with Rabbit anti-PHD2 antibody at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of PHD2 on different lysates with Rabbit anti-PHD2 antibody at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | PHD2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human PHD2 aa 151-426. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | EGLN1 |
|---|---|
| Gene Synonyms | HPH2 PHD2 SM20 ECYT3 HALAH HPH-2 HIFPH2 ZMYND6 C1orf12 HIF-PH2 |
| Gene Full Name | egl-9 family hypoxia inducible factor 1 |
| Gene Summary | The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). [provided by RefSeq, Nov 2009] |
| Alternative Names | C1ORF12 antibody Chromosome 1 Open Reading Frame 12 antibody DKFZp761F179 antibody ECYT 3 antibody ECYT3 antibody Egl 9 family hypoxia inducible factor 1 antibody EGL 9 homolog of C. elegans 1 antibody EGL nine (C.elegans) homolog 1 antibody Egl nine homolog 1 (C. elegans) antibody Egl nine homolog 1 antibody C1ORF12 antibody Chromosome 1 Open Reading Frame 12 antibody DKFZp761F179 antibody ECYT 3 antibody ECYT3 antibody Egl 9 family hypoxia inducible factor 1 antibody EGL 9 homolog of C. elegans 1 antibody EGL nine (C.elegans) homolog 1 antibody Egl nine homolog 1 (C. elegans) antibody Egl nine homolog 1 antibody Egl nine like protein 1 antibody EGLN 1 antibody Egln1 antibody EGLN1_HUMAN antibody HIF PH2 antibody HIF Prolyl Hydroxylase 2 antibody HIF-PH2 antibody HIF-prolyl hydroxylase 2 antibody HIFP4H 2 antibody HIFPH2 antibody HPH 2 antibody HPH-2 antibody HPH2 antibody Hypoxia inducible factor prolyl hydroxylase 2 antibody Hypoxia-inducible factor prolyl hydroxylase 2 antibody ORF13 antibody P4H2 antibody PHD 2 antibody PhD2 antibody PNAS 118 antibody PNAS 137 antibody Prolyl Hydroxylase Domain Containing Protein 2 antibody Prolyl hydroxylase domain-containing protein 2 antibody Rat Homolog of SM20 antibody SM 20 antibody SM-20 antibody SM20 antibody Zinc finger MYND domain containing protein 6 antibody ZMYND6 antibody |
| Molecular Weight(MW) | 46 kDa(Observed band size: 50 kDa) |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of PHD2 on different lysates with Rabbit anti-PHD2 antibody at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of PHD2 on different lysates with Rabbit anti-PHD2 antibody at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
