WB
Western blot analysis of PHD2/EGLN1 using anti-PHD2/EGLN1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PHD2/EGLN1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
ICC/IF analysis of PHD2/EGLN1 using anti-PHD2/EGLN1 antibody. PHD2/EGLN1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-PHD2/EGLN1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).IP
IP analysis of PHD2/EGLN1 using anti-PHD2/EGLN1 antibody in A549 whole cell lysate. Western blot analysis of PHD2/EGLN1 using anti- PHD2/EGLN1 antibody. Lane 1: A549 whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- PHD2/EGLN1 antibody in A549 whole cell lysate, Lane 3: anti- PHD2/EGLN1 antibody (2μg) + A549 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- PHD2/EGLN1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a mouse anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.FC
Flow Cytometry analysis of SH-SY5Y cells using anti-PHD2/EGLN1 antibody. Overlay histogram showing SH-SY5Y cells stained with anti-PHD2/EGLN1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PHD2/EGLN1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to PHD2/EGLN1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human PHD2/EGLN1. Human PHD2/EGLN1 shares 100% amino acid (aa) sequence identity with both mouse and rat PHD2/EGLN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIPWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 IP:1:250-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EGLN1 |
|---|---|
| Gene Synonyms | HPH2 PHD2 SM20 ECYT3 HALAH HPH-2 HIFPH2 ZMYND6 C1orf12 HIF-PH2 |
| Gene Full Name | egl-9 family hypoxia inducible factor 1 |
| Gene Summary | The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). [provided by RefSeq, Nov 2009] |
| Alternative Names | EGLN1 PHD2 HIF-PH2 EGLN 1 ECYT3 |
| Molecular Weight(MW) | 46 kDa |
| Cellular Localization | Cytoplasm,Nucleus |
WB
Western blot analysis of PHD2/EGLN1 using anti-PHD2/EGLN1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PHD2/EGLN1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
ICC/IF analysis of PHD2/EGLN1 using anti-PHD2/EGLN1 antibody. PHD2/EGLN1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-PHD2/EGLN1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IP
IP analysis of PHD2/EGLN1 using anti-PHD2/EGLN1 antibody in A549 whole cell lysate. Western blot analysis of PHD2/EGLN1 using anti- PHD2/EGLN1 antibody. Lane 1: A549 whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- PHD2/EGLN1 antibody in A549 whole cell lysate, Lane 3: anti- PHD2/EGLN1 antibody (2μg) + A549 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- PHD2/EGLN1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a mouse anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
FC
Flow Cytometry analysis of SH-SY5Y cells using anti-PHD2/EGLN1 antibody. Overlay histogram showing SH-SY5Y cells stained with anti-PHD2/EGLN1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PHD2/EGLN1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 IP:1:250-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
