Application
Immunofluorescence analysis of methanol-fixed HeLa, using PHGDH antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using PHGDH antibody at 1:500 dilution.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000Application
Sample(30 μg of whole cell lysate) A:293T B:A431 C:HeLa S3 7.5% SDS PAGE diluted at 1:1000Application
PHGDH antibody [N1N2], N-term detects PHGDH protein at cytoplasm on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. PHGDH antibody [N1N2], N-term diluted at 1:500.Application
Immunoprecipitation of PHGDH protein from 293T whole cell extracts using 5 μg of PHGDH antibody [N1N2], N-term . Western blot analysis was performed using PHGDH antibody [N1N2], N-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | PHGDH antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PHGDH (phosphoglycerate dehydrogenase)3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human PHGDH. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Chimpanzee, BovineRat (97%), Pig (97%), Chimpanzee (100%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 3-PGDH antibody 3PGDH antibody MGC3017 antibody PDG antibody PGAD antibody PGD antibody PGDH antibody SERA antibody PHGDH antibody 3-phosphoglycerate dehydrogenase antibody D-3-phosphoglycerate dehydrogenase antibody phosphoglycerate dehydrogena |
|---|---|
| Molecular Weight(MW) | 57 kDa (note) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using PHGDH antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using PHGDH antibody at 1:500 dilution.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000
Application
Sample(30 μg of whole cell lysate) A:293T B:A431 C:HeLa S3 7.5% SDS PAGE diluted at 1:1000
Application
PHGDH antibody [N1N2], N-term detects PHGDH protein at cytoplasm on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. PHGDH antibody [N1N2], N-term diluted at 1:500.
Application
Immunoprecipitation of PHGDH protein from 293T whole cell extracts using 5 μg of PHGDH antibody [N1N2], N-term . Western blot analysis was performed using PHGDH antibody [N1N2], N-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | 293T , A431 , HeLaS3 , mouse brain , *SK-N-DZ |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
