Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using PHGDH antibody at 1:250 dilution.Application
Sample (30 ug of whole cell lysate) A: A431 B: Raji 7.5% SDS PAGE diluted at 1:10000| Product Name | PHGDH antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PHGDH (phosphoglycerate dehydrogenase)3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PHGDH. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Chimpanzee, BovineMouse (96%), Rat (97%), Pig (90%), Chimpanzee (100%), Bovine (83%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 3-PGDH antibody 3PGDH antibody MGC3017 antibody PDG antibody PGAD antibody PGD antibody PGDH antibody SERA antibody PHGDH antibody 3-phosphoglycerate dehydrogenase antibody D-3-phosphoglycerate dehydrogenase antibody phosphoglycerate dehydrogena |
|---|---|
| Molecular Weight(MW) | 57 kDa (note) |
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using PHGDH antibody at 1:250 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431 B: Raji 7.5% SDS PAGE diluted at 1:10000| Positive Control | 293T , A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
