Application
Western blot analysis of anti-PHLPP1 Antibody (N-term) in K562 cell line lysates (35ug/lane).PHLPP1 (arrow) was detected using the purified Pab.| Product Name | PHLPP1 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PHLPP1 (N-term)Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-66' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and suppression of tumor growth. Controls the phosphorylation of AKT2 and AKT3 more efficiently than that of AKT1. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. May act as a negative regulator of K-Ras signaling in membrane rafts. |
| Immunogen | KLH conjugated synthetic peptide between 26~56 amino acids from the N-terminal region of human PHLPP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | PH domain leucinerich repeatcontaining protein phosphatase EC 3.1.3.16 PHLPP Suprachiasmatic nucleus circadian oscillatory protein EC3.1.3.16 PLEKHE1 SCOP PH domain leucinerich repeat protein phosphatase PHLPP1 Pleckstrin homology domain-containin |
|---|---|
| Target | PHLPP1 |
| Molecular Weight(MW) | 184.672 kDa (note) |
| Cellular Localization | In colorectal cancer tissue, expression is concentrated at the lateral membrane of epithelial cells. |
Application
Western blot analysis of anti-PHLPP1 Antibody (N-term) in K562 cell line lysates (35ug/lane).PHLPP1 (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
