Application
PHLPP2 Antibody (N-term) western blot analysis in HL-60 cell line lysates (15ug/lane).This demonstrates the PHLPP2 antibody detected the PHLPP2 protein (arrow).| Product Name | PHLPP2 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PHLPP2 (N-term)Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-66' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. |
| Immunogen | KLH conjugated synthetic peptide between 11~40 amino acids from the N-term region of human PHLPP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | PH domain leucine-rich repeat protein phosphatase-like PHLPP-like PHLPPlike KIAA0931 PHLPPL PH domain leucinerich repeat protein phosphataselike PHLPP2 PHLPP like |
|---|---|
| Target | PHLPP2 |
| Molecular Weight(MW) | 146.751 kDa (note) |
| Cellular Localization | In colorectal cancer tissue, expression is concentrated in the cytoplasm and nucleus. |
Application
PHLPP2 Antibody (N-term) western blot analysis in HL-60 cell line lysates (15ug/lane).This demonstrates the PHLPP2 antibody detected the PHLPP2 protein (arrow).| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
