Application
Whole cell extract of rat brain cortex was separated on SDS-PAGE and blotted with Monoclonal Anti-Phosphoserine (Product No. ). The antibody was developed with Goat Anti-Mouse IgG, Biotin Conjugate , ExtrAvidin, Peroxidase Conjugate and AEC substrate.| Product Name | Phosphoserine antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [PSR - 45] to PhosphoserineThis antibody has been used in immunoblotting (ref 1) for the localization of some phosphoserine-containing proteins. |
| Immunogen | Recombinant fragment: (pS) conjugated to KLH. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | PSR-45 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| The antibody reacts specifically with phosphorylated serine, both as free amino acid or conjugated to carriers such as BSA or KLH. Indirect ELISA (1:4,000)Immunoblotting (colorimetric, 1:500): |
|
| Species Reactivity | MouseOther |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Phosphoserine |
|---|---|
| Tissue Specificity | Monoclonal Anti-Phosphoserine reacts against phosphorylated serine both as free amino acid or when conjugated to carriers such as BSA or KLH using ELISA and dot blot. No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. |
Application
Whole cell extract of rat brain cortex was separated on SDS-PAGE and blotted with Monoclonal Anti-Phosphoserine (Product No. ). The antibody was developed with Goat Anti-Mouse IgG, Biotin Conjugate , ExtrAvidin, Peroxidase Conjugate and AEC substrate.| Application Notes | The antibody reacts specifically with phosphorylated serine, both as free amino acid or conjugated to carriers such as BSA or KLH. Indirect ELISA (1:4,000)Immunoblotting (colorimetric, 1:500): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA |
