Application
Human breast carcinoma stained with Immunohistochemistry (FFPE-sections).| Product Name | Phospho-serine/threonine/tyrosine antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [SPM101] to Phosphoserine / threonine / tyrosineSerine, threonine, and tyrosine are amino acids. Protein phosphorylation provides a signalling system that can be thought of as a kind of protein on/off switch. |
| Immunogen | Phosphoserine, phosphothreonine, and phosphotyrosine conjugated to protein carriers. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | SPM101 |
| Host Species | Mouse |
| Tested Applications | IHC-PIPWB |
| For ELISA: Use at an assay dependent dilution. For IF: Use at an assay dependent dilution. For IHC-P: Use at a dilution of 1:20 for 10 minutes at RT. Requires boiling tissue sections in 10 mM citrate, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. For IP: Use at a concentration of 2 μg/ml. For WB: Use at a concentration of 1-2 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Other |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Target | Phosphoserine/threonine/tyrosine |
|---|---|
| Tissue Specificity | recognises serine, threonine, and tyrosine phosphorylated proteins. It may cross-react with other phosphorylated amino acids. |
| Cellular Localization | Cell Surface and Cytoplasmic |
Application
Human breast carcinoma stained with Immunohistochemistry (FFPE-sections).| Positive Control | Breast carcinoma |
|---|---|
| Application Notes | For ELISA: Use at an assay dependent dilution. For IF: Use at an assay dependent dilution. For IHC-P: Use at a dilution of 1:20 for 10 minutes at RT. Requires boiling tissue sections in 10 mM citrate, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. For IP: Use at a concentration of 2 μg/ml. For WB: Use at a concentration of 1-2 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing BSA and sodium azide |
