| Product Name | Phosphotyrosine antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [1.BB.49] to PhosphotyrosineThe role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. |
| Immunogen | Phosphotyrosine coupled to carrier protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 1.BB.49 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHCIPWB |
| For ELISA: Use at a dilution of 1:2,000. For ICC: Use at a dilution of 1:500. For IP: Use at a dilution of 1:500. For WB: Use at a dilution of 1:2,000. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Other |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | Phosphotyrosine |
|---|---|
| Tissue Specificity | Recognizes phosphorylated tyrosine residues, and does not crossreact with phosphorylated threonine or phosphorylated serine residues. |
| Application Notes | For ELISA: Use at a dilution of 1:2,000. For ICC: Use at a dilution of 1:500. For IP: Use at a dilution of 1:500. For WB: Use at a dilution of 1:2,000. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 3mM sodium azide and 50% glycerol |
