| Product Name | Phosphotyrosine antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [120.1] to PhosphotyrosineCellular regulation of enzyme activity involves a reversible covalent modification of the enzymes. In many cases this modification is achieved by the phosphorylation of specific serine, threonine or tyrosine residue in the enzyme. This reaction is catalyzed by a family of protein kinases that transfer phosphate from ATP to specific residues. |
| Immunogen | Phosphotyrosine conjugated to KLH. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 120.1 |
| Host Species | Mouse |
| Tested Applications | DotELISAWB |
| Slotblot, immunoblotting 1:100. ELISA 1:200-1:400. Optimal working dilutions and conditions should be determined individually by the laboratory. Applicability of this antibody for immunohistochemistry on routinely processed paraffin-embedded or cryostat tissue sections has not been established.: |
|
| Species Reactivity | Other |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Phosphotyrosine |
|---|---|
| Tissue Specificity | This antibody detects free and conjugated phosphotyrosine as well as phosphotyrosine in modified cellular proteins. It does not cross-react with nonphosphorylated tyrosine, phosphothreonine, phosphoserine, AMP or ATP. Due to steric hindrance, certain proteins known to contain phosphorylated tyrosine may not be recognized by this antibody. |
| Positive Control | For immunoblotting we recommend extract of fresh human platelet |
|---|---|
| Application Notes | Slotblot, immunoblotting 1:100. ELISA 1:200-1:400. Optimal working dilutions and conditions should be determined individually by the laboratory. Applicability of this antibody for immunohistochemistry on routinely processed paraffin-embedded or cryostat tissue sections has not been established.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 20 mM tris-borate, 150 mM sodium chloride, BMC-6 carrier polysaccarides, carrier protein, and 0.1% sodium azide, pH 7.5 |
