| Product Name | Phosphotyrosine antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [2Q267] to PhosphotyrosineThe role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. |
| Immunogen | Chemical / Small Molecule (Hapten O-phopshotyramine) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 2Q267 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIPWB |
| ELISA: Use at an assay dependent dilution. ICC: Use at a concentration of 5 - 10 μg/ml. IP: Use at a concentration of 100 μg/ml. WB: Use at a concentration of 0.4 - 1 μg/ml. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Other |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Target | Phosphotyrosine |
|---|---|
| Tissue Specificity | Binds specifically and with a high affinity to proteins with phosphorylated tyrosine residues. Binding can be blocked by phosphotyrosine and phenyl phosphate (IC50:10e-6M, ELISA). Does not crossreact with tyrosine, phosphoserine, phosphothreonine, sulphotyrosine (sulfated (23-25) cholecystokinine) or Na-benzoate |
| Application Notes | ELISA: Use at an assay dependent dilution. ICC: Use at a concentration of 5 - 10 μg/ml. IP: Use at a concentration of 100 μg/ml. WB: Use at a concentration of 0.4 - 1 μg/ml. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide. Constituents: 0.25M Sodium chloride, 0.02M PBS, pH 7.6 |
