| Product Name | Phosphotyrosine antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [3G239] to PhosphotyrosineThe role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. |
| Immunogen | KLH coupled with equal amounts of phosphotyrosine, glycine, and alanine. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 3G239 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| IP: Use at a concentration of 2 - 5 μg/ml. WB: Use at a concentration of 0.5 - 1 μg/ml. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Other |
| Concentration | 0.2mg/ml |
| Purification | Protein A |
| Target | Phosphotyrosine |
|---|---|
| Tissue Specificity | Reacts specifically with tyrosine phosphorylated proteins in the native or denatured states. It does not react with phosphoserine or phosphothreonine. |
| Application Notes | IP: Use at a concentration of 2 - 5 μg/ml. WB: Use at a concentration of 0.5 - 1 μg/ml. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide. Constituents: 10mM Sodium phosphate. pH 7.4 |
