Application
Phosphotyrosine pattern in HeLa cells as revealed by P-Tyr-01 antibody -Alexa Fluor® 488 (green). Actin detected by phalloidin-Alexa Fluor® 567, DNA stained by DAPI (blue).| Product Name | Phosphotyrosine antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [P-Tyr-01] to PhosphotyrosineDetection of activation-induced tyrosine phosphorylation by Western Blotting. |
| Immunogen | Phosphotyrosine conjugated to BSA. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | P-Tyr-01 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| ICC: Use at an assay dependant dilution. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Other |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | Phosphotyrosine |
|---|---|
| Tissue Specificity | Phosphotyrosine. |
Application
Phosphotyrosine pattern in HeLa cells as revealed by P-Tyr-01 antibody -Alexa Fluor® 488 (green). Actin detected by phalloidin-Alexa Fluor® 567, DNA stained by DAPI (blue).| Positive Control | Jurkat cells stimulated with anti CD3 antibody (2 min incubation) |
|---|---|
| Application Notes | ICC: Use at an assay dependant dilution. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
