| Product Name | PHOX2B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PHOX2BPhox2b (paired-like homeobox 2b, neuroblastoma paired-type homeobox protein) is a transcription factor involved in the development of several major noradrenergic neuron populations and in the determination of neurotransmitter phenotypes. |
| Immunogen | Synthetic peptide: PGSCSLGTLRDHQSSP conjugated to BSA, corresponding to amino acids 62-77 of Human PHOX2B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| For IHC: Use at a dilution of 1:200 - 1:1,000. For WB: Use at a dilution of 1:200 - 1:1,000. Predicted molecular weight: 31.6 kDa. Method 1 for immunostaining and immunoblotting (Western blot): 1. Carefully resuspend antibody pellet to uniformity. 2. Remove a fixed amount of suspension and dissolve 1/5 in PBS or TBS to yield a 100 μg/ml solution. Method 2 for immunoprecipitation, supershift, immunostaining and immunoblotting (Western blot): 1. Pellet antibodies at 10,000 - 15,000 x g for 10 minutes at 2 to 8°C using a microcentrifuge.2. Carefully remove as much supernatant as possible. It is not necessary to remove all the ammonium sulfate solution a small residual amount will not effect the antibody preparation. Dissolve the pellet (antibody) in small volume (50 μL) of PBS (or TBS) at final concentration of 1 mg/ml (50 μg/50 μl). Do not allow the pellet to dry out. This can cause loss of activity. Gently allow pellet to dissolve at least 1 hour before use. Do not vortex. Mix by finger-tapping or gentle stirring. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | NBLST2 NBPhox PMX2B |
|---|---|
| Target | PHOX2B |
| Cellular Localization | Nuclear |
| Application Notes | For IHC: Use at a dilution of 1:200 - 1:1,000. For WB: Use at a dilution of 1:200 - 1:1,000. Predicted molecular weight: 31.6 kDa. Method 1 for immunostaining and immunoblotting (Western blot): 1. Carefully resuspend antibody pellet to uniformity. 2. Remove a fixed amount of suspension and dissolve 1/5 in PBS or TBS to yield a 100 μg/ml solution. Method 2 for immunoprecipitation, supershift, immunostaining and immunoblotting (Western blot): 1. Pellet antibodies at 10,000 - 15,000 x g for 10 minutes at 2 to 8°C using a microcentrifuge.2. Carefully remove as much supernatant as possible. It is not necessary to remove all the ammonium sulfate solution a small residual amount will not effect the antibody preparation. Dissolve the pellet (antibody) in small volume (50 μL) of PBS (or TBS) at final concentration of 1 mg/ml (50 μg/50 μl). Do not allow the pellet to dry out. This can cause loss of activity. Gently allow pellet to dissolve at least 1 hour before use. Do not vortex. Mix by finger-tapping or gentle stirring. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline and 50% ammonium sulfate |
