Flow Cytometry: phycoerythrin Antibody - C57BL/6 splenocytes stained with RM4-5 PE and purified phycoerythrin antibody , then detected with anti-mouse IgG1 FITC.
Mouse Monoclonal antibody [PE001] to PhycoerythrinThe PE001 antibody reacts with both phycoerythrin (PE) alone and PE conjugated with antibody or streptavidin, as well as PE tandem dyes such as PE/Cy5, PE/Cy5.5 and PE/Cy7. When PE001 antibody is used in a three-step staining procedure or separation of PE-antibody labeled cells, it does not quench PE fluorescence or react with tested mouse and human cells. The PE001 antibody can be used for separation of PE-antibody labeled cells or as a secondary step to amplify PE conjugated primary antibody signals (i.e. biotinylated PE001 as secondary step, followed by Streptavidin-PE).
Immunogen
Phycoerythrin
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
PE001
Host Species
Mouse
Tested Applications
FACS
FACS: 1:10 - 1:1000:
Species Reactivity
All
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Target
Phycoerythrin
Tissue Specificity
phycoerythrin (PE001)
Application
Application
Flow Cytometry: phycoerythrin Antibody - C57BL/6 splenocytes stained with RM4-5 PE and purified phycoerythrin antibody , then detected with anti-mouse IgG1 FITC.
Application Notes
FACS: 1:10 - 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Store at 4C. Do not freeze
Storage Buffer
PBS (pH 7.2) and 0.09% sodium azide was added as a preservative.