Application
ELISA analysis of control antigen and antigen using PIK3CA antibody [4F3].Application
FACS analysis of Jurkat cells using PIK3CA antibody [4F3] (green) or a negative control (red).Application
ICC/IF analysis of HeLa cells using PIK3CA antibody [4F3] (green) and DRAQ5 fluorescent DNA dye (blue).Application
WB analysis of lysates from (1) untransfected and (2) PIK3CA (a.a. 881-1068)-hIgGFc transfected HEK293 cells using PIK3CA antibody [4F3].Application
WB analysis of human PIK3CA (a.a. 881-1068) recombinant protein using PIK3CA antibody [4F3].| Product Name | PI3 kinase p110 alpha antibody [4F3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [4F3] to PIK3CAPhosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human PIK3CA expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4F3 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFWB |
| ELISA: 1/10000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | MCMTC , P110-ALPHA , CWS5 , PI3K , MCM , CLOVE , MCAP |
|---|---|
| Target | PIK3CA |
| Molecular Weight(MW) | 124 kDa (note) |
Application
ELISA analysis of control antigen and antigen using PIK3CA antibody [4F3].
Application
FACS analysis of Jurkat cells using PIK3CA antibody [4F3] (green) or a negative control (red).
Application
ICC/IF analysis of HeLa cells using PIK3CA antibody [4F3] (green) and DRAQ5 fluorescent DNA dye (blue).
Application
WB analysis of lysates from (1) untransfected and (2) PIK3CA (a.a. 881-1068)-hIgGFc transfected HEK293 cells using PIK3CA antibody [4F3].
Application
WB analysis of human PIK3CA (a.a. 881-1068) recombinant protein using PIK3CA antibody [4F3].| Application Notes | ELISA: 1/10000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer. |
