Application
ELISA analysis of control antigen and antigen using PIK3R1 antibody [6G10].Application
FACS analysis of NIH3T3 cells using PIK3R1 antibody [6G10] (green) or a negative control (red).Application
ICC/IF analysis of HeLa cells using PIK3R1 antibody [6G10] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).Application
ICC/IF analysis of HepG2 cells using PIK3R1 antibody [6G10] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).Application
IHC-P analysis of cervical cancer tissues using PIK3R1 antibody [6G10].Application
IHC-P analysis of rectum cancer tissues using PIK3R1 antibody [6G10].Application
WB analysis of human PIK3R1 recombinant protein using PIK3R1 antibody [6G10].| Product Name | PI3 kinase p85 alpha antibody [6G10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [6G10] to PI3 kinase p85 alphaPhosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011] |
| Immunogen | The immunogen corresponding to a region within amino acids 159 and 388 of human PIK3R1 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6G10 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | P85-ALPHA , P85 , GRB1 |
|---|---|
| Target | PIK3R1 |
| Molecular Weight(MW) | 83.6 kDa (note) |
Application
ELISA analysis of control antigen and antigen using PIK3R1 antibody [6G10].
Application
FACS analysis of NIH3T3 cells using PIK3R1 antibody [6G10] (green) or a negative control (red).
Application
ICC/IF analysis of HeLa cells using PIK3R1 antibody [6G10] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Application
ICC/IF analysis of HepG2 cells using PIK3R1 antibody [6G10] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Application
IHC-P analysis of cervical cancer tissues using PIK3R1 antibody [6G10].
Application
IHC-P analysis of rectum cancer tissues using PIK3R1 antibody [6G10].
Application
WB analysis of human PIK3R1 recombinant protein using PIK3R1 antibody [6G10].| Application Notes | ELISA: 1/10000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer. |
