| Product Name | PI3 kinase p85 alpha antibody [8-2D-4D] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [8 - 2D - 4D] to PI3 kinase p85 alphaThe enzyme PI-3-K phosphorylates at the D-3 position of the inositol ring of PI, PI-4- phosphate, and PI-4,5-biphosphate (PI-4,5-P2) to produce their respective PI3-phosphorylated derivatives. PI-3-K exists as a heterodimer of 85 kDa (p85) and 110 kDa (p110) subunits. The p85 subunit contains two SH2 domains and an SH3 domain. It has been shown to associate with and serve as a substrate for activated growth factor receptor tyrosine kinases. p85 may serve as regulator of the catalytic subunit, p110, by acting as the link between PI-3-K and the ligand-activated receptor. Two distinct forms of the p85 subunit are described: p85 alpha, which binds tightly to the catalytic subunit and p85 beta, a protein of unknown function. Both isoforms bind to activated receptors and serve as tyrosine kinase substrates. |
| Immunogen | Purified fragment (aa 333 - 430) of recombinant human PI-3-K p85 alpha subunit. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 8-2D-4D |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| For IF: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Detects a band of ~ 85 kDa, which corresponds to the predicted molecular weight of PI 3 Kinase. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | GRB1, p85, p85-ALPHA, p85 ALPHA, p85ALPHA |
|---|---|
| Target | PI 3 Kinase p85 alpha |
| Cellular Localization | Cytoplasmic |
| Application Notes | For IF: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Detects a band of ~ 85 kDa, which corresponds to the predicted molecular weight of PI 3 Kinase. Optimal dilutions/concentrations should be determined by the researcher. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10 mM Phosphate-buffered saline, pH 7.4, 0.2%BSA, 0.09% sodium azide |
