Application
PI3 kinase p85 alpha antibody [N2C1], Internal immunoprecipitates PI3 kinase p85 alpha protein in IP experiments. IP samples: Jurkat whole cell extract A. Control with 5 μg of preimmune Rabbit IgG B. Immunoprecipitation of PI3 kinase p85 alpha protein by 5 μg PI3 kinase p85 alpha antibody [N2C1], Internal 10 % SDS-PAGE The immunoprecipitated PI3 kinase p85 alpha protein was detected by PI3 kinase p85 alpha antibody [N2C1], Internal diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PI3 kinase p85 alpha antibody [N2C1], Internal detects PIK3R1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. PI3 kinase p85 alpha antibody [N2C1], Internal diluted at 1:500.| Product Name | PI3 kinase p85 alpha antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PI3 kinase p85 alpha (phosphoinositide-3-kinase, regulatory subunit 1 (alpha))Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PI3 kinase p85 alpha. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| WB:1:500~1:2000 IP:1:100~1:500 IHC-p:1:50~1:500 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (99%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GRB1 antibody p85 antibody p85-ALPHA antibody PIK3R1 antibody phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody |
|---|---|
| Molecular Weight(MW) | 84 kDa (note) |
Application
PI3 kinase p85 alpha antibody [N2C1], Internal immunoprecipitates PI3 kinase p85 alpha protein in IP experiments. IP samples: Jurkat whole cell extract A. Control with 5 μg of preimmune Rabbit IgG B. Immunoprecipitation of PI3 kinase p85 alpha protein by 5 μg PI3 kinase p85 alpha antibody [N2C1], Internal 10 % SDS-PAGE The immunoprecipitated PI3 kinase p85 alpha protein was detected by PI3 kinase p85 alpha antibody [N2C1], Internal diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PI3 kinase p85 alpha antibody [N2C1], Internal detects PIK3R1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. PI3 kinase p85 alpha antibody [N2C1], Internal diluted at 1:500.| Positive Control | Jurkat , K562 , THP-1 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 |
|---|---|
| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IHC-p:1:50~1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
