Application
ICC/IF analysis of HepG2 cells using PINCH antibody [5G7] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).Application
WB analysis of (1) A549, (2) Jurkat and (3) HeLa lysates using PINCH antibody [5G7].Application
WB analysis of human PINCH (a.a. 87-249) recombinant protein using PINCH antibody [5G7].Application
ELISA analysis of control antigen and antigen using PINCH antibody [5G7].Application
FACS analysis of Hela cells using PINCH antibody [5G7] (blue) or a negative control (red).| Product Name | PINCH antibody [5G7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [5G7] to PINCHThe protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010] |
| Immunogen | The immunogen corresponding to human PINCH expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5G7 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFWB |
| ELISA: 1/10000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PINCH-1 , LIMS1 , PINCH1 |
|---|---|
| Target | PINCH |
| Molecular Weight(MW) | 37 kDa (note) |
Application
ICC/IF analysis of HepG2 cells using PINCH antibody [5G7] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Application
WB analysis of (1) A549, (2) Jurkat and (3) HeLa lysates using PINCH antibody [5G7].
Application
WB analysis of human PINCH (a.a. 87-249) recombinant protein using PINCH antibody [5G7].
Application
ELISA analysis of control antigen and antigen using PINCH antibody [5G7].
Application
FACS analysis of Hela cells using PINCH antibody [5G7] (blue) or a negative control (red).| Application Notes | ELISA: 1/10000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
