Application
Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using anti-Pirh2 mouse mAbApplication
Western blot analysis using Pirh2 mAb against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate.Application
Flow cytometric analysis of PC-12 cells using anti-Pirh2 mAb (blue) and negative control (red).Application
Immunofluorescence analysis of Hela cells using Pirh2 mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.| Product Name | Pirh2 antibody [1H10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1H10] to Pirh2The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including TP53, HDAC1 and CDKN1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011] |
| Immunogen | The immunogen corresponding to human Pirh2 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1H10 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHC-PWB |
| ELISA: 1/10000. | |
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Gene Synonyms | HARNIP , CHIMP , PRO1996 , RNF199 , PIRH2E , ZNF363 , ARNIP , PIRH2F , RCHY1 , PIRH2 |
|---|---|
| Target | Pirh2 |
| Molecular Weight(MW) | 30 kDa; 60 kDa (homodimer) (note) |
Application
Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using anti-Pirh2 mouse mAb
Application
Western blot analysis using Pirh2 mAb against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate.
Application
Flow cytometric analysis of PC-12 cells using anti-Pirh2 mAb (blue) and negative control (red).
Application
Immunofluorescence analysis of Hela cells using Pirh2 mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.| Application Notes | ELISA: 1/10000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
