Application
Anti-PRKAR1A mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PRKAR1AApplication
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PRKAR1A mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-PRKAR1Amouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-PRKAR1Amouse monoclonal antibody. ( Dilution 1:50)Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKAR1A monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PRKAR1Amouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded colon tissue using anti-PRKAR1Amouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-PRKAR1Amouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR1A.| Product Name | PKA I alpha reg antibody [6C7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [6C7] to PKA I alpha regcAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Three alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq] |
| Immunogen | Protein expressed in 293T cell transfected with human PRKAR1A expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6C7 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-PWB |
| IHC 1:50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PRKAR1A, CAR, PPNAD1, MGC17251, TSE1, CNC, CNC1, PKR1, PRKAR1, DKFZp779L0468 |
|---|---|
| Target | PRKAR1A |
| Tissue Specificity | is specific for human PRKAR1A. |
Application
Anti-PRKAR1A mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PRKAR1A
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PRKAR1A mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-PRKAR1Amouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-PRKAR1Amouse monoclonal antibody. ( Dilution 1:50)
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKAR1A monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PRKAR1Amouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded colon tissue using anti-PRKAR1Amouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-PRKAR1Amouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR1A.| Application Notes | IHC 1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
