Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKC delta (Phospho-Ser645) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from PC12 cells untreated or treated with PMA using PKC delta (Phospho-Ser645) Antibody .Application
Western blot analysis of extracts from PC12 cells untreated or treated with PMA using PKCd (Phospho-Ser645) Antibody| Product Name | PKC delta (phospho Ser645) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKC delta (Phospho Ser645)This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin. |
| Immunogen | Peptide sequence around phosphorylation site of serine 645 (R-L-S(p)-Y-S) derived from Human PKC delta . |
| Modification | p-Ser645 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 2012/5/1, MGC49908, PKCD, nPKC-delta, PRKCD, protein kinase C delta type, protein kinase C delta VIII, protein kinase C, delta |
|---|---|
| Target | PKC delta (Phospho Ser645) |
| Molecular Weight(MW) | 78 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKC delta (Phospho-Ser645) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from PC12 cells untreated or treated with PMA using PKC delta (Phospho-Ser645) Antibody .
Application
Western blot analysis of extracts from PC12 cells untreated or treated with PMA using PKCd (Phospho-Ser645) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
