Application
Immunofluorescence staining of methanol-fixed Hela cells using PKC delta Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKC delta Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from PC12 cells using PKC delta Antibody .Application
Western blot analysis of extracts from PC12 cells using PKCd (Ab-645) Antibody| Product Name | PKC delta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKC deltaThis is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol. which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin. |
| Immunogen | Peptide sequence around aa. 643~647 (R-L-S-Y-S) derived from Human PKC delta . |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 2012/5/1, MGC49908, PKCD, nPKC-delta, PRKCD, protein kinase C delta type, protein kinase C delta VIII, protein kinase C, delta |
|---|---|
| Target | PKC delta |
| Molecular Weight(MW) | 78 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using PKC delta Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKC delta Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from PC12 cells using PKC delta Antibody .
Application
Western blot analysis of extracts from PC12 cells using PKCd (Ab-645) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
