Application
Cell lysates (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PKM2 (1:500, ). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Application
Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human PKM2 antibody (1:500, ) with Texas Red (Red).| Product Name | PKM2 [AT4M3] antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [AT4M3] to PKM2 |
| Immunogen | Recombinant human PKM2 (1-531aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | AT4M3 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| Recommended Starting Dilutions: For WB: Use at a dilution of 1:250 - 500. For ICC/IF: Use at a dilution of 1:250 - 500. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | PKM2 |
|---|
Application
Cell lysates (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PKM2 (1:500, ). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Application
Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human PKM2 antibody (1:500, ) with Texas Red (Red).| Application Notes | Recommended Starting Dilutions: For WB: Use at a dilution of 1:250 - 500. For ICC/IF: Use at a dilution of 1:250 - 500. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide |
