Application
Flow cytometric analysis of Hela cells, using anti-PKMYT1 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
Flow cytometric analysis of Jurkat cells, using anti-PKMYT1 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
Anti-PKMYT1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PKMYT1Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKMYT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKMYT1.| Product Name | PKMYT1 antibody [2B4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2B4] to PKMYT1Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development. |
| Immunogen | Full length human recombinant protein of human PKMYT1 (NP_004194) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 2B4 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100, | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MYT1, DKFZp547K1610, PKMYT1 |
|---|---|
| Target | PKMYT1 |
| Tissue Specificity | is specific for human PKMYT1 (NP_004194). |
Application
Flow cytometric analysis of Hela cells, using anti-PKMYT1 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
Flow cytometric analysis of Jurkat cells, using anti-PKMYT1 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
Anti-PKMYT1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PKMYT1
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKMYT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKMYT1.| Application Notes | ICC/IF 1:100, |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
