Application
WB analysis of LNCap, HeLa, human placenta and A549 lysates using PLAP antibody at a dilution of 1:500.Application
ICC/IF analysis of HeLa cells using PLAP antibody at a dilution of 1:300.| Product Name | Placental Alkaline Phosphatase antibody [8F10-2C10-C9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [8F10-2C10-C9] to Placental alkaline phosphatase (PLAP)There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3´ untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. |
| Immunogen | Purified recombinant human PLAP protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 8F10-2C10-C9 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PLAP , ALPP , ALP , alkaline phosphatase, placental , PALP , PLAP-1 |
|---|---|
| Target | Placental alkaline phosphatase (PLAP) |
| Molecular Weight(MW) | 76kDa (note) |
| Cellular Localization | Cell membrane; Lipid-anchor ＞ GPI-anchor |
Application
WB analysis of LNCap, HeLa, human placenta and A549 lysates using PLAP antibody at a dilution of 1:500.
Application
ICC/IF analysis of HeLa cells using PLAP antibody at a dilution of 1:300.| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide, 50% glycerol, 0.1%BSA |
