Rabbit polyclonal to Human placental lactogenPlacental lactogen is a member of the somatotropin/prolactin family of hormones and plays an important role in growth control. The gene is located at the growth hormone locus on chromosome 17 along with four other related genes in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed mainly in the placenta and utilizes multiple transcription initiation sites.
Immunogen
Purified human placental lactogen
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHC-PWB
IHC-P: Use at a dilution of 1/250 for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. WB: Use at an assay dependent dilution. Predicted molecular weight: 25 kDa. Optimal dilutions/concentrations should be determined by the end user.
Species Reactivity
Human
Concentration
0.2mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
CSA, CSH2, CSMT, FLJ75407, PL
Target
Placental lactogen
Cellular Localization
Secreted
Application
Application
Formalin-fixed, paraffin-embedded human placenta stained with using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of trophoblasts
Positive Control
Placenta
Application Notes
IHC-P: Use at a dilution of 1/250 for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. WB: Use at an assay dependent dilution. Predicted molecular weight: 25 kDa. Optimal dilutions/concentrations should be determined by the end user.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Storage Buffer
PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.