| Product Name | Plakophilin 1 antibody [3G250] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [3G250] to Plakophilin 1Plays a role in formation of desmosomal plaques and is found in desmosomes of most simple and stratified epithelia. Not found in cell types that have non-epithelial desmosomes. Absent in fibroblasts and other connective tissue types, including sarcomas. |
| Immunogen | Plakophilin 1 (Human) (N terminal). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3G250 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIPWB |
| ELISA: Use at a dilution of 1/100 - 1/1000. IF: Use at a concentration of 3 - 10 μg/ml. IP: Use 2μg per IP reaction. WB: Use at a concentration of 1 - 3 μg/ml. Predicted molecular weight: 87 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein A |
| Gene Synonyms | B6P, Plakophilin1, Plakophilin 1, MGC138829 |
|---|---|
| Target | Plakophilin 1 |
| Tissue Specificity | Reacts with the N-terminus of human plakophilin 1. |
| Cellular Localization | Nuclear |
| Positive Control | MCF-7 cell lysates |
|---|---|
| Application Notes | ELISA: Use at a dilution of 1/100 - 1/1000. IF: Use at a concentration of 3 - 10 μg/ml. IP: Use 2μg per IP reaction. WB: Use at a concentration of 1 - 3 μg/ml. Predicted molecular weight: 87 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | May be stored at 4ºC for short term only. For long term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20ºC. Aliquots are stable for at least 12 months at -20ºC. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: PBS. pH 7.2 |
