| Product Name | PLK1 antibody [Mixed clones] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [Mixed clones] to PLK1Polo-like kinases are a family of serine/threonine protein kinases, named after the prototypic founding member of the family, the polo gene product of Drosophila melanogaster. The polo kinase was originally identified in mutants that display abnormal mitotic spindle organization. Subsequently, potential homologues of Drosophila polo have been identified in yeasts (Cdc5p in Saccharomyces cerevisiae; plo1+ in Schizosaccharmoyces pombe) and in mammals (polo-like kinase 1; Plk1). Genetic and biochemical studies suggest that polo, Cdc5p and plo1+ may be required for mitotic spindle organization and, possibly, for cytokinesis. Likewise, the patterns of expression, activity and subcellular localization of Plk1 suggest that Plk12 may function during mitosis in spindle assembly and function. Recent studies have demonstrated that mammalian Plks regulate the function of the Golgi complex, a cellular organelle closely associated with the centrosome and also have microtubule organization activity. Furthermore, deregulated expression of human PLK1 and PLK3 is strongly correlated with the development of many types of malignancies, and ectopic expression of kinase-active Plk3 or Plk1 dominant negative protein leads to rapid cell death. |
| Immunogen | Fusion protein: DPACIPIFWVS KWVDYSDKYG LGYQLCDNS VGVLFNDST RLILYNDGDSL QYIERDGTESY LTVSSHPNSLM KKITLLKYFRN YMSEHLLKAGA NITPREGDELA RLPYLRTWF RTRSAIILH LSNGSVQINFF QDHTKLILCP LMAAVTYIDE KRDFRTYRLS LLEEYGCCKE LASRLRYARTM VDKLLSSRSAS NRLKAS, corresponding to |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | Mixed clones |
| Host Species | Mouse |
| Tested Applications | ELISA,ICC/IF,IP,WB |
| ELISA: Use at a concentration of 0.1 - 1 μg/ml. IF: Use at a concentration of 2 - 5 μg/ml. IP: Use at a concentration of 2 - 5 μg/ml. WB: Use at a concentration of 1 - 2 μg/ml. Detects a band of approximately 68 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human,Mouse,Pig,Rat,Xenopus |
| Concentration | 0.2mg/ml |
| Purification | Protein A |
| Gene Synonyms | PLK, STPK13 |
|---|---|
| Target | PLK1 |
| Tissue Specificity | This antibody reacts specifically with the 68 kDa Plk1 protein. However, cross-reactivity with a 110 kDa protein of unknown identity is sometimes observed. |
| Cellular Localization | Nuclear |
| Light Chain Type | kappa |
| Application Notes | ELISA: Use at a concentration of 0.1 - 1 μg/ml. IF: Use at a concentration of 2 - 5 μg/ml. IP: Use at a concentration of 2 - 5 μg/ml. WB: Use at a concentration of 1 - 2 μg/ml. Detects a band of approximately 68 kDa. Optimal dilutions/concentrations should be determined by the end user. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 40% Glycerol, PBS. pH 7.4 |
