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IF: Use at a dilution of 1/500 - 1/5000. WB: Use at a dilution of 1/10000 (cell lysates, ECL detection). For non-ECL western detection methods, use at a dilution of 1/1000 - 1/5000. Also detects Pma1p-containing fusion proteins on western blots. Predicted molecular weight: 100 kDa. Optimal dilutions/concentrations should be determined by the end user. |