BALB/C mice were injected with P. carinii cysts isolated from human lung.
Key Feature
Clonality
Monoclonal
Isotype
IgM
Clone Number
3F6
Host Species
Mouse
Tested Applications
IHC-F,IHC-P
This antibody may be diluted to a titer of 1:10-1:50 in an ABC method. We suggest an incubation period of 30-60 minutes at room temperature. Enzymatic predigestion with proteolytic enzymes can increase the staining in paraffin embedded formalin fixed tissue sections. Optimal dilutions/concentrations should be determined by the researcher.
Concentration
1mg/ml
Purification
Unpurified
Target Information
Target
Pneumocystis carinii
Tissue Specificity
The antibody reacts with a 82 kDa polypeptide specific to P. carinii. It does not cross-react with G. lamblia, T. gondii, T. cruzi, L. tropica, E. histolytica, C. albicans and P. falciparum.
Cellular Localization
Alveolar spaces
Light Chain Type
kappa
Positive Control
Infected lung
Application Notes
This antibody may be diluted to a titer of 1:10-1:50 in an ABC method. We suggest an incubation period of 30-60 minutes at room temperature. Enzymatic predigestion with proteolytic enzymes can increase the staining in paraffin embedded formalin fixed tissue sections. Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.