Mouse monoclonal antibody [AT1C11] to PNMTThe product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]
Immunogen
Recombinant human PNMT (1-282aa) purified from E. coli
Key Feature
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
AT1C11
Host Species
Mouse
Tested Applications
ELISAWB
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500.
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
WB analysis of Jurkat lysate (40ug) using PNMT antibody at a dilution of 1:500.
Application Notes
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500.
Additional Information
Form
Liquid
Storage Instructions
Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
Storage Buffer
Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide