Application
PNPase antibody [4C11] detects PNPase protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PNPase antibody [4C11] diluted at a dilution of 1:1000.| Product Name | PNPase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4C11] to PNPaseThis antibody binds to PNPase1. PNPase, or PNPT1, is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation (Raijmakers et al., 2002 [PubMed 12419256]).[supplied by OMIM]. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4C11 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| Suggested starting dilutions are as follows: WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp762K1914 old-35 OLD35 PNPASE PNPT1 old 35 |
|---|---|
| Target | PNPase |
Application
PNPase antibody [4C11] detects PNPase protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PNPase antibody [4C11] diluted at a dilution of 1:1000.| Positive Control | Jurkat , Raji , K562 , THP-1 |
|---|---|
| Application Notes | Suggested starting dilutions are as follows: WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short term use, store at 4°C. For extended use, aliquot and store at -20°C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS |
