Application
Anti-Podoplanin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/mlApplication
The extracts of mouse kidney (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDPN (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Application
Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with PDPN antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody LS-D2, alkaline phosphatase-streptavidin and chromogen.Application
Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with PDPN antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody LS-D2, alkaline phosphatase-streptavidin and chromogen.| Product Name | Podoplanin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5E2] to PodoplaninThis gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq] |
| Immunogen | Recombinant human Podoplanin (1-206aa) purified from E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 5E2 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-PWB |
| WB (1:500 - 1:1000), ELISA, IHC-P (5 µg/ml): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | AGGRUS GP36 GP40 Gp38 HT1A-1 OTS8 PA2.26 T1A T1A-2 T1A 2 HT1A 1 T1A2 HT1A1 |
|---|
Application
Anti-Podoplanin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml
Application
The extracts of mouse kidney (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDPN (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Application
Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with PDPN antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody LS-D2, alkaline phosphatase-streptavidin and chromogen.
Application
Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with PDPN antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody LS-D2, alkaline phosphatase-streptavidin and chromogen.| Application Notes | WB (1:500 - 1:1000), ELISA, IHC-P (5 µg/ml): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, pH 7.4, 0.1% sodium azide |
