Rabbit Polyclonal antibody to POFUT1This gene encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Immunogen
POFUT1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human POFUT1.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAIHC-PWB
WB: 0.5 - 1 ug/ml. IHC-P: 2.5 ug/ml:
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
O-FucT-1 FUT12 O-FUT O-Fuc-T protein O-fucosyltransferase 1
Target
POFUT1
Tissue Specificity
At least two isoforms of POFUT1 are known to exist. This antibody is predicted to not cross-react with POFUT2.
Application
Application
Western blot analysis of POFUT1 in human heart tissue lysate with POFUT1 antibody at (A) 0.5 and (B) 1 ug/mL.
Application
Immunohistochemistry of POFUT1 in human heart tissue with POFUT antibody at 2.5 ug/mL.
Application Notes
WB: 0.5 - 1 ug/ml. IHC-P: 2.5 ug/ml:
Additional Information
Form
Liquid
Storage Instructions
Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.