| Product Name | Polo like kinase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [AZ27] to Polo like kinaseSmith et al. (1997) stated that PLK localizes to the mitotic spindle, suggesting that PLK is involved in regulating mitotic spindle function. Smith et al. (1997) showed that microinjection of PLK mRNA induced mitosis in quiescent NIH 3T3 cells. Constitutive expression of PLK caused NIH 3T3 cells to proliferate in low serum media, but at a lower rate than cells transformed with v-Ras or v-Src. Cells transformed with PLK grew in soft agar and produced tumors in nude mice. Smith et al. (1997) concluded that PLK may be involved in the promotion or progression of cancers. |
| Immunogen | Recombinant full length protein (Xenopus laevis). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | AZ27 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| ELISA: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. WB: Use at a concentration of 1 μg/ml. Detects a band of approximately ~55 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseXenopus |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | MGC52566 plk |
|---|---|
| Target | Polo like kinase |
| Tissue Specificity | The cross-reactivity of this antibody regards the various isoforms of PLK is not known. |
| Application Notes | ELISA: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. WB: Use at a concentration of 1 μg/ml. Detects a band of approximately ~55 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: NoneConstituents: PBS |
