Application
Western blot analysis of PPAPDC1A in human brain tissue lysate with PPAPDC1A antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of PPAPDC1A in human brain tissue with PPAPDC1A antibody at 20 μg/mL.| Product Name | PPAPDC1A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PPAPDC1APhosphatidate phosphatase (PAP) plays important role in lipid-signaling metabolism in eukaryotic cells. Two distinct types of PAP (PAP1 and PAP2) activity have been distinguished by their subcellular localization and differential sensitivity to N-ethylmaleimide(NEM) and Mg2+. A yeast diacylglycerol pyrophosphate (DGPP) phosphatase (DPP1) and mammalian DGPP phosphatase (PAP2) have been identified as Mg2+-independent and NEM-insensitive membrane-associated. PPAPDC1A (also known as DPPL2) and PPAPDC1B (DPPL1) form a novel type of Mg2+-independent and NEM-sensitive mammalian phosphatidate phosphatase showing broad substrate specificity. PPAPDC1A is preferentially expressed in endothelial cells. Studies of PPAPDC1A and PAP activity suggest that they may play a role in angiogenesis. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DPPL2 phosphatidic acid phosphatase type2 domain containing1 PPAPDC1A phosphatidic acid phosphatase type 2 domain containing 1 PPAPDC1 |
|---|---|
| Target | PPAPDC1A |
| Tissue Specificity | This antibody is specific for PPAPDC1A |
Application
Western blot analysis of PPAPDC1A in human brain tissue lysate with PPAPDC1A antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of PPAPDC1A in human brain tissue with PPAPDC1A antibody at 20 μg/mL.| Positive Control | Blocking Peptide: |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
